analýza polymorfismu délky amplifikovaných restrikčních fragmentů
in situ značení DNA s primerem
kvantitativní polymerázová řetězová reakce
multiplexová polymerázová řetězová reakce
polymerázová řetězová reakce s reverzní transkripcí
technika náhodné amplifikace polymorfní DNA
ligasová řetězová reakce (RT)
místa se sekvenční adresou (RT)
Linking entry
Polymerase Chain Reaction
Conspect
E05. - 393. - 620. - 500
Note
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Database
MESH
References
(8) - MESH
subject heading
Number of the records: 1
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