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Decoding the Antibody Repertoire
Title statement Decoding the Antibody Repertoire [electronic resource] : High Throughput Sequencing of Multiple Transcripts from Single B Cells / by Brandon DeKosky. Publication Cham : Springer International Publishing : Imprint: Springer, 2017. Phys.des. XXVIII, 87 p. 34 illus. online resource. ISBN 9783319585185 Edition Springer Theses, Recognizing Outstanding Ph.D. Research, ISSN 2190-5053 Contents Background -- High-throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire -- In-Depth Determination and Analysis of the Human Paired Heavy and Light Chain Antibody Repertoire -- Paired VH:VL Analysis of Naïve B Cell Repertoires and Comparison to Antigen-Experienced B Cell Repertoires in Healthy Human Donors -- Conclusions and Future Perspectives -- Appendices. Notes to Availability Přístup pouze pro oprávněné uživatele Another responsib. SpringerLink (Online service) Subj. Headings Medicine. * Human genetics. * Antibodies. * Genetic engineering. * Biochemistry. * Cell biology. Form, Genre elektronické knihy electronic books Country Německo Language angličtina Document kind Electronic books URL Plný text pro studenty a zaměstnance UPOL 
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This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening the door for the discovery of new antibodies and the investigation of adaptive immune responses to vaccines and diseases. By designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells, the author directly addresses the limitations to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. Previous methods for high-throughput immune repertoire sequencing have been unable to provide such information. The techniques developed in this thesis have enabled comprehensive investigation of human B-cell repertoires and have been applied for the rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.
Background -- High-throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire -- In-Depth Determination and Analysis of the Human Paired Heavy and Light Chain Antibody Repertoire -- Paired VH:VL Analysis of Naïve B Cell Repertoires and Comparison to Antigen-Experienced B Cell Repertoires in Healthy Human Donors -- Conclusions and Future Perspectives -- Appendices.
Number of the records: 1