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A study of molecular and cellular activities of brassinosteroids and their derivatives

  1. Title statementA study of molecular and cellular activities of brassinosteroids and their derivatives [rukopis] / Lucie Hoffmannová
    Additional Variant TitlesStudium molekulárních a bunecných úcinku brassinosteroidu a jejich derivátu
    Personal name Hoffmannová, Lucie (dissertant)
    Translated titleA study of molecular and cellular activities of brassinosteroids and their derivatives
    Issue data2010
    Phys.des.41 s. (67 490 zn.), 95 s. příl : il., grafy, schémata, tab. + 1 CD ROM
    NoteVed. práce Miroslav Strnad
    Another responsib. Strnad, Miroslav, 1958- (thesis advisor)
    Hlobilková, Alice (opponent)
    Kovařík, Jan (opponent)
    Aa-neuveden, -- (opponent)
    Another responsib. Univerzita Palackého. Katedra botaniky (degree grantor)
    Keywords brassinosteroid * angiogenesis * antiproliferative * cancer cell lines * endothelial cells * immunoblotting * indirect immunofluorescence * flow cytometry * steroid receptor * brassinosteroid * angiogenesis * antiproliferative * cancer cell lines * endothelial cells * immunoblotting * indirect immunofluorescence * flow cytometry * steroid receptor
    Form, Genre disertace dissertations
    UDC (043.3)
    CountryČesko
    Languageangličtina
    Document kindPUBLIKAČNÍ ČINNOST
    TitlePh.D.
    Degree programDoktorský
    Degree programBiologie
    Degreee disciplineBotanika
    book

    book

    Kvalifikační práceDownloadedSizedatum zpřístupnění
    130025-215872175.pdf4710.5 MB24.06.2010
    Call numberBarcodeLocationSublocationInfo
    DIS/085 (PřF-KBO)3134512125PřF-HolicePřF, Knihovna Holice - skladIn-Library Use Only

    Molecular and cellular effects of two groups of antiproliferative agents, natural brassinosteroids and their synthetic derivatives, were examined in different adherent human cancer cell lines (MCF-7, MDA-MB-468) and in primary endothelial cells (HUVEC, HMEC-1). Their effects were studied by classical biochemical methods (SDS-polyacrylamide gel electrophoresis and immunoblotting, indirect immunofluorescence analysis, flow cytometry, caspase activation assay) and by angiogenesis assays in vitro (proliferation, migration, tube formation). Brassinosteroids and their derivatives can inhibit the growth of several human cancer cell lines and inhibit angiogenesis-like behaviour of primary endothelial cells in vitro.Molecular and cellular effects of two groups of antiproliferative agents, natural brassinosteroids and their synthetic derivatives, were examined in different adherent human cancer cell lines (MCF-7, MDA-MB-468) and in primary endothelial cells (HUVEC, HMEC-1). Their effects were studied by classical biochemical methods (SDS-polyacrylamide gel electrophoresis and immunoblotting, indirect immunofluorescence analysis, flow cytometry, caspase activation assay) and by angiogenesis assays in vitro (proliferation, migration, tube formation). Brassinosteroids and their derivatives can inhibit the growth of several human cancer cell lines and inhibit angiogenesis-like behaviour of primary endothelial cells in vitro.

Number of the records: 1  

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