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A study of molecular and cellular activities of brassinosteroids and their derivatives
Title statement A study of molecular and cellular activities of brassinosteroids and their derivatives [rukopis] / Lucie Hoffmannová Additional Variant Titles Studium molekulárních a bunecných úcinku brassinosteroidu a jejich derivátu Personal name Hoffmannová, Lucie (dissertant) Translated title A study of molecular and cellular activities of brassinosteroids and their derivatives Issue data 2010 Phys.des. 41 s. (67 490 zn.), 95 s. příl : il., grafy, schémata, tab. + 1 CD ROM Note Ved. práce Miroslav Strnad Another responsib. Strnad, Miroslav, 1958- (thesis advisor) Hlobilková, Alice (opponent) Kovařík, Jan (opponent) Aa-neuveden, -- (opponent) Another responsib. Univerzita Palackého. Katedra botaniky (degree grantor) Keywords brassinosteroid * angiogenesis * antiproliferative * cancer cell lines * endothelial cells * immunoblotting * indirect immunofluorescence * flow cytometry * steroid receptor * brassinosteroid * angiogenesis * antiproliferative * cancer cell lines * endothelial cells * immunoblotting * indirect immunofluorescence * flow cytometry * steroid receptor Form, Genre disertace dissertations UDC (043.3) Country Česko Language angličtina Document kind PUBLIKAČNÍ ČINNOST Title Ph.D. Degree program Doktorský Degree program Biologie Degreee discipline Botanika 
book
Kvalifikační práce Downloaded Size datum zpřístupnění 130025-215872175.pdf 53 10.5 MB 24.06.2010 Call number Barcode Location Sublocation Info DIS/085 (PřF-KBO) 3134512125 PřF-Holice PřF, Knihovna Holice - sklad In-Library Use Only
Molecular and cellular effects of two groups of antiproliferative agents, natural brassinosteroids and their synthetic derivatives, were examined in different adherent human cancer cell lines (MCF-7, MDA-MB-468) and in primary endothelial cells (HUVEC, HMEC-1). Their effects were studied by classical biochemical methods (SDS-polyacrylamide gel electrophoresis and immunoblotting, indirect immunofluorescence analysis, flow cytometry, caspase activation assay) and by angiogenesis assays in vitro (proliferation, migration, tube formation). Brassinosteroids and their derivatives can inhibit the growth of several human cancer cell lines and inhibit angiogenesis-like behaviour of primary endothelial cells in vitro.Molecular and cellular effects of two groups of antiproliferative agents, natural brassinosteroids and their synthetic derivatives, were examined in different adherent human cancer cell lines (MCF-7, MDA-MB-468) and in primary endothelial cells (HUVEC, HMEC-1). Their effects were studied by classical biochemical methods (SDS-polyacrylamide gel electrophoresis and immunoblotting, indirect immunofluorescence analysis, flow cytometry, caspase activation assay) and by angiogenesis assays in vitro (proliferation, migration, tube formation). Brassinosteroids and their derivatives can inhibit the growth of several human cancer cell lines and inhibit angiogenesis-like behaviour of primary endothelial cells in vitro.
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