Cílem této diplomové práce je zjistit vliv prostředí na objemovou a elektrogenerovanou chemiluminiscenci luminolu. Byly proměřeny závislosti CL a ECL luminolu na pH v různých pufrech (TRIS, fosfátový pufr) a závislost reakce na přídavcích peroxidu vodíku, dále vliv přídavku CTAB. CL luminolu byla startována buď běžným iniciačním systémem Co2+?H2O2 nebo elektrochemickou cestou. Při měření ECL byl nalezen výrazný rozdíl při experimentech prováděných na platinové elektrody a elektrodě ze skelného uhlíku. V práci je zkoumán vliv pracovní elektrody a CTAB na ECL.The chemiluminescence (CL) of luminol and its analogues (derivatives) is one of the most studied CL reactions, which is often used in imunoassay, detection of ROS (Reactive oxygen species) and in medicine. It is a sensitive analytical technique which is used in many branches. CL can proceed by two reaction pathways leading to light emsision. In the submitted diploma thesis it is investigated the effect of TRIS on the chemiluminescence of luminol. CL and electrogenerated chemiluminescence (ECL) of luminol were measured in two different buffers (TRIS and phosphate buffer). The chemiluminescence was initiated by Co2+?H2O2 or electrochemical method. Platinum electrode and glassy carbon electrode were used to produce ECL. Light intensity was measured in depence on pH and concentration hydrogen peroxide. We demonstrate the effect of TRIS buffer on the intensity of CL and ECL. When compared the identical luminol CL and ECL experiments conducted in TRIS and phosphate buffer of the same pH, the CL and ECL of luminol was different in these buffers, especially in the presence of hydrogen peroxide CTAB.