Cieľom tejto práce, bolo transformovať Arabidopsis thaliana konštruktmi pomocou Agrobacterium tumefaciens a vyselektovať rastliny s T-DNA. V rámci práce boli vytvorené heterozygotné semená T1 generácie, ktoré obsahujú konštrukty DU.GUS a IU.GUS a semená T2 generácie s konštruktom DGU.US. Ďalším predmetom tejto práce bolo zistiť, či sa gén dpcr3-2 podieľa na homológnej rekombinácii. Podľa teórie pokiaľ sa gén dpcr3-2 podiela na HR, tak by sa v mutantovi malo vyskytovať menej modrých bodiek, ktoré je možné pozorovať v mladých listoch Arabidopsis thaliana po histochemickom farbení, ako vo WT. WT totižto nemá funkčný gén a teda homológna rekombinácia nemôže prebiehať rovnako efektívne ako v mutantovi. Analýzou frekvencie homológnej rekombinácie nebolo možné s istotou určiť či sa gén dpcr3-2 podieľa na homológnej rekombinácii.The aim of the thesis was to transform Arabidopsis thaliana with constructs using Agrobacterium tumefaciens and select plants with T-DNA. As part of the work, heterozygous seeds of T1 generation were created and contain the constructs DU.GUS and IU.GUS and also seeds of T2 generation with DGU.US construct were created. Another subject of this work was to determin whether the dpcr3-2 gene is involved in homologous recombination. According to the theory, if the dpcr3-2 gene participates in HR, the mutant should have fewer blue dots, which can be observed in young leaves of Arabidopsis thaliana after histochemical staining, than in WT. WT does not have a functional gene and therefore homologou recombination cannot take place as efficiently as in the mutant. By analyzing the frequency of homologous recombination, it was not possible to determine with certainty wheter the dpcr3-2 gene is involved in homologou recombination.