Bakalárska práca je zameraná na popis ochorenia IgA nefropatia a jeho príčiny, na popis glykozylácie pántovej oblasti imunoglobulínu A1 a štruktúry tohto imunoglobulínu, na vývoj B lymfocytov v ľudskom organizme a na vplyv interleukínu-6 na rozvoj IgA nefropatie. Experimentálna časť je venovaná charakterizácii buniek z periférnej krvi. Vyizolované mononukleárne bunky z periférnej krvi boli stimulované interleukínom-6 a následne bol určovaný ich fenotyp pomocou povrchového farbenia a metódy prietokovej cytometrie. Bol tak simulovaný stav zvýšenia hladiny interleukínu-6 v ľudskom organizme. Určené fenotypy boli porovnané s fenotypmi nestimulovaných buniek a bol zhodnotený vplyv interleukínu-6 na vývoj B lymfocytov a jeho vplyv na ochorenie IgA nefropatia. Pozornosť bola venovaná aj metóde prietokovej cytometrie a bolo porovnané meranie klasickej prietokovej cytometrie a meranie spektrálnej cytometrie.The bachelor thesis is focused on the description of IgA nephropathy and its cause, on the description of glycosylation of the hinge region of immunoglobulin A1 and the structure of this immunoglobulin, on the development of B lymphocytes in the human body and on the influence of interleukin-6 on the development of IgA nephropathy. The experimental part is devoted to the characterization of peripheral blood cells. Isolated peripheral blood mononuclear cells were stimulated by interleukin-6 and their phenotype was determined by surface staining and flow cytometry method. The situation of interleukin-6 increased level in the human body was simulated. The determined phenotypes were compared with unstimulated cell phenotypes and the effect of interleukin-6 on B cell development and on IgA nephropathy was evaluated. Attention was also paid to the method of flow cytometry and the measurement of the classical flow cytometry and the measurement of the spectral cytometry were compared.