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Oxidative stress in higher plants studied by novel experimental approaches
Title statement Oxidative stress in higher plants studied by novel experimental approaches [rukopis] / Aditya Kumar Additional Variant Titles Oxidative stress in higher plants studied by novel experimental approaches Personal name Kumar, Aditya, (dissertant) Translated title Oxidative stress in higher plants studied by novel experimental approaches Issue data 2020 Phys.des. 55 + Publications I. Kumar, A., Prasad, A., Sedlárová, M., Pospíšil, P. (2019). Organic radical imaging in plants: focus on protein radicals. Free Radical Biology and Medicine, 130, 568-575. II. Kumar, A., Prasad, A., Sedlářová, M., Pospíšil, P. (2018). Data on detection of singlet oxygen, hydroxyl radical and organic radical in Arabidopsis thaliana. Data in Brief, 21, 2246-2252. III. Kumar, A., Prasad, A., Sedlářová, M., Pospíšil, P. (2019). Characterization of Protein Radicals in Arabidopsis. Frontiers in Physiology, 10, 958. IV. Prasad, A., Kumar, A., Suzuki, M., Kikuchi, H., Sugai, T., Kobayashi, M., Pospíšil, P., Tada, M., & Kasai, S. (2015). Detection of hydrogen peroxide in Photosystem II (PSII) using catalytic amperometric biosensor. Frontiers in Plant Science, 6, 862. V. Prasad, A., Kumar, A., Matsuoka, R., Takahashi, A., Fujii, R., Sugiura, Y., Kikuchi, H., Aoyagi, S., Aikawa, T., Kondo, T., Yuasa, M., Pospíšil, P., Kasai, S. (2017). Real-time monitoring of superoxide anion radical generation in response to wounding: electrochemical study. PeerJ, 5, e3050. VI. Kasai, S., Sugiura, Y., Prasad, A., Inoue, K., Sato, T., Honmo, T., Kumar, A., Pospíšil, P., Ino, K., Hashi, Y., Furubayashi, Y., Matsudaira, M., Suda, A., Kunikata, R., Matsue, T. (2019). Real-time imaging of photosynthetic oxygen evolution from spinach using LSI-based biosensor. Scientific Reports, 9, 12234 Note Ved. práce Pavel Pospíšil Another responsib. Pospíšil, Pavel (školitel) Another responsib. Univerzita Palackého. Katedra biofyziky (degree grantor) Keywords biosensor * electrochemical techniques * light * oxidative stress * photosynthesis * photosystem II * protein oxidation * reactive oxygen species * biosensor * electrochemical techniques * light * oxidative stress * photosynthesis * photosystem II * protein oxidation * reactive oxygen species Form, Genre disertace dissertations UDC (043.3) Country Česko Language angličtina Document kind PUBLIKAČNÍ ČINNOST Title Ph.D. Degree program Doktorský Degree program Fyzika Degreee discipline Biofyzika book
Kvalifikační práce Downloaded Size datum zpřístupnění 00219632-444945547.pdf 15 13.8 MB 15.06.2020 Posudek Typ posudku 00219632-ved-244294074.pdf Posudek vedoucího 00219632-opon-135577934.pdf Posudek oponenta
Proteins are the major target for reactive oxygen species as a result of their abundance in biological systems. Stress conditions induce the upsurge in the level of reactive oxygen species causing protein oxidation recognized by the formation of protein radicals, fragments, aggregates, and subsequent degradation. In the current study, we used the immuno-spin trapping technique for the detection of protein radicals in vivo and in vitro. We performed western blotting for the characterization of proteins involved in the formation of protein radicals, fragments, and aggregates. We observed that light stress triggered the upsurge in the level of hydroxyl radical and singlet oxygen. Hydroxyl radical and singlet oxygen induced protein oxidation, which is recognized by the formation of reactive carbon-centered (alkyl) and oxygen-centered (peroxyl and alkoxyl) protein radicals. Generation and kinetic behavior of reactive oxygen species and molecular oxygen in spinach leaves have been studied under abiotic stress. We used electrochemical biosensor techniques for the real-time monitoring of superoxide anion radical and hydrogen peroxide formation and two-dimensional imaging of light-induced oxygen evolution. Immuno-spin trapping technique for the detection and characterization of protein radical formation is imperative for better understating of the mechanism of oxidative modification in PSII proteins under high light stress and electrochemical biosensor techniques can offer precise information on the qualitative determination of superoxide anion radical, hydrogen peroxide and molecular oxygen.Proteins are the major target for reactive oxygen species as a result of their abundance in biological systems. Stress conditions induce the upsurge in the level of reactive oxygen species causing protein oxidation recognized by the formation of protein radicals, fragments, aggregates, and subsequent degradation. In the current study, we used the immuno-spin trapping technique for the detection of protein radicals in vivo and in vitro. We performed western blotting for the characterization of proteins involved in the formation of protein radicals, fragments, and aggregates. We observed that light stress triggered the upsurge in the level of hydroxyl radical and singlet oxygen. Hydroxyl radical and singlet oxygen induced protein oxidation, which is recognized by the formation of reactive carbon-centered (alkyl) and oxygen-centered (peroxyl and alkoxyl) protein radicals. Generation and kinetic behavior of reactive oxygen species and molecular oxygen in spinach leaves have been studied under abiotic stress. We used electrochemical biosensor techniques for the real-time monitoring of superoxide anion radical and hydrogen peroxide formation and two-dimensional imaging of light-induced oxygen evolution. Immuno-spin trapping technique for the detection and characterization of protein radical formation is imperative for better understating of the mechanism of oxidative modification in PSII proteins under high light stress and electrochemical biosensor techniques can offer precise information on the qualitative determination of superoxide anion radical, hydrogen peroxide and molecular oxygen.
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