Methods
Methods in protein biochemistry [elektronický zdroj] / edited by Harald Tschesche. -- Berlin : De Gruyter, 2011. -- 1 online zdroj (xxvi, 378 s.) : il., portréty. -- 9.3 Cofactors in biology provide spectroscopic access to reaction cycles.. -- Popsáno podle tištěné verze. -- Obsahuje: Preface; Editor; List of contributing authors; Abbreviations; Acknowledgements; 1 Three-phase partitioning; 1.1 Method; 1.2 The mechanism of TPP; 1.3 A practical example -- the isolation of cathepsin L from liver tissue; 1.4 Other applications; 2 Folding and degradation functions of molecular chaperones; 2.1 Introduction; 2.2 The domain structure of Hsc/Hsp70; 2.3 The Hsc/Hsp70 reaction cycle; 2.4 Cochaperones determine the function of Hsc/Hsp70; 2.5 In vitro reconstitution and functional analysis of the Hsc/Hsp70 chaperone system; 2.6 Measuring the ATPase activity of Hsc/Hsp70. -- Resumé: This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in s. -- ISBN : 9783110252361 (e-book). -- 3110252368 (e-book).
Tschesche, Harald
biochemie. bílkoviny. proteomika. chemické zbraně. elektronické knihy
577.1. 577.112. 623.459. 577.112.01:575.111. (0.034.2:08)