Directed
Directed enzyme evolution [elektronický zdroj] : screening and selection methods / edited by Frances H. Arnold and George Georgiou. -- Totowa, N.J. : Humana Press, c2003. -- xv, 383 s. : il. (některé barev.). -- (Methods in molecular biology ISSN 1064-3745 ; vol. 230). -- Resumé: Seasoned practitioners from many leading laboratories describe their best readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens. An accompanying volume, Directed Evolution Library Creation: Methods and Protocols (ISBN 1-58829-285-1), describes readily reproducible methods for the creation of mutated DNA molecules and DNA libraries. Copy for Both Volumes Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function. -- ISBN : 9781592593965 (e-book).
Arnold, Frances Hamilton, 1956-. Georgiou, George, 1959-
biotechnologie. enzymy. molekulární evoluce. laboratorní práce. elektronické knihy
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