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In vitro models of drug resistance in oncology
Údaje o názvu In vitro models of drug resistance in oncology [rukopis] / Michaela Sapíková Další variantní názvy In vitro modely lékové rezistence v onkologii Osobní jméno Sapíková, Michaela, (autor diplomové práce nebo disertace) Překl.náz In vitro models of drug resistance in oncology Vyd.údaje 2023 Fyz.popis 63 s : grafy, schémata, tab. Poznámka Oponent Tomáš Oždian Ved. práce Lenka Řeháčková Dal.odpovědnost Oždian, Tomáš, (oponent) Řeháčková, Lenka, (vedoucí diplomové práce nebo disertace) Dal.odpovědnost Univerzita Palackého. Katedra buněčné biologie a genetiky (udelovatel akademické hodnosti) Klíč.slova Drug resistance * nucleosides * oncology * in vitro models * Drug resistance * nucleosides * oncology * in vitro models Forma, žánr bakalářské práce bachelor's theses MDT (043)378.22 Země vyd. Česko Jazyk dok. angličtina Druh dok. PUBLIKAČNÍ ČINNOST Titul Bc. Studijní program Bakalářský Studijní program Biologie Studijní obor Molekulární a buněčná biologie kniha
Kvalifikační práce Staženo Velikost datum zpřístupnění 00283201-973682064.pdf 0 1.7 MB 04.05.2025 Posudek Typ posudku 00283201-ved-869235461.pdf Posudek vedoucího 00283201-opon-890657821.pdf Posudek oponenta
This bachelor's thesis deals with the development and basic characterization of cell lines resistant to nucleoside cytostatics, cultivated in vitro. The theoretical part of the work is devoted to general facts associated with the development of cancer and mechanisms of resistance to cytostatic drugs, focusing on nucleoside derivatives that are used in clinical practice for the treatment of leukaemia and solid tumours. The literature review also highlights the importance of in vitro models in drug development. The beginning of the experimental part of the work is focused on the selection of resistant cells. Resistance was verified using the MTS cytotoxicity test, furthermore, the screening of new potential drugs is involved. The second part is dedicated to their basic characterization using standardized procedures for this laboratory. The expression of P-glycoprotein and MRP1 was analyzed by flow cytometry. The presence of proteins associated with the mTOR pathway was determined by western blotting.This bachelor's thesis deals with the development and basic characterization of cell lines resistant to nucleoside cytostatics, cultivated in vitro. The theoretical part of the work is devoted to general facts associated with the development of cancer and mechanisms of resistance to cytostatic drugs, focusing on nucleoside derivatives that are used in clinical practice for the treatment of leukaemia and solid tumours. The literature review also highlights the importance of in vitro models in drug development. The beginning of the experimental part of the work is focused on the selection of resistant cells. Resistance was verified using the MTS cytotoxicity test, furthermore, the screening of new potential drugs is involved. The second part is dedicated to their basic characterization using standardized procedures for this laboratory. The expression of P-glycoprotein and MRP1 was analyzed by flow cytometry. The presence of proteins associated with the mTOR pathway was determined by western blotting.
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