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Novel electrochemical sensors for analysis of biological active compounds
Údaje o názvu Novel electrochemical sensors for analysis of biological active compounds [rukopis] / Soodabeh Hassanpour Další variantní názvy Nové elektrochemické senzory pro analýzu biologicky aktivních sloučenin Osobní jméno Hassanpour, Soodabeh, (autor diplomové práce nebo disertace) Překl.náz Novel electrochemical sensors for analysis of biological active compounds Vyd.údaje 2023 Fyz.popis 108 : il., grafy, schémata, tab. Poznámka Ved. práce Jan Petr Dal.odpovědnost Petr, Jan (vedoucí diplomové práce nebo disertace) Dal.odpovědnost Univerzita Palackého. Katedra analytické chemie (udelovatel akademické hodnosti) Klíč.slova (Bio)sensors * Screen-printed electrodes * Disposable * Dasatinib * Single-walled carbon nanotubes * Horseradish peroxidase * Hydrogen peroxide * Molecularly imprinted polymer * Poly(tyrosine)-Chitosan * Bioelectrocatalyst * Genosensor * Haemophilus influenza * DNA bio-assay * citrate capped silver nanoparticle * (Bio)sensors * Screen-printed electrodes * Disposable * Dasatinib * Single-walled carbon nanotubes * Horseradish peroxidase * Hydrogen peroxide * Molecularly imprinted polymer * Poly(tyrosine)-Chitosan * Bioelectrocatalyst * Genosensor * Haemophilus influenza * DNA bio-assay * citrate capped silver nanoparticle Forma, žánr disertace dissertations MDT (043.3) Země vyd. Česko Jazyk dok. angličtina Druh dok. PUBLIKAČNÍ ČINNOST Titul Ph.D. Studijní program Doktorský Studijní program Analytical Chemistry Studijní obor Analytical Chemistry kniha
Kvalifikační práce Staženo Velikost datum zpřístupnění 00282345-675508686.pdf 2 4.8 MB 02.06.2023 Posudek Typ posudku 00282345-opon-184995551.pdf Posudek oponenta 00282345-ved-268961401.pdf Posudek vedoucího 00282345-opon-178284396.pdf Posudek oponenta Průběh obhajoby datum zadání datum odevzdání datum obhajoby přidělená hodnocení typ hodnocení 00282345-prubeh-915451114.pdf 16.03.2021 02.06.2023 29.08.2023 S Hodnocení známkou
Recently, medical organizations and research disciplines have switched to employing affordable biosensors. Biosensors have become more critical in drug development, drug identification, bio-medicine, food safety, security, protection, and ecological research. It has directly contributed to developing specialized and reliable diagnostic equipment that uses biological sensing components as biosensors. Different analytes, biological receptors, and transducer concepts are used in biosensors. In recent years, innovative sensing platforms have been developed employing screen-printed electrodes (SPEs), which are inexpensive, simple, and quick mass production through thick film technology. During my Ph.D., I concentrated on designing, constructing, and validating several SPE-based (bio)sensors to identify biologically active compounds important for healthcare applications, demonstrating their significant potential as a suitable sensing platform. I created (bio)sensors for a variety of analytes, from nucleic acid to small molecules, with specific modification strategies to endow the platforms with selectivity toward the species of interest. I developed disposable electrochemical (bio)sensors in two papers based on modifying with single-walled carbon nanotubes (SWCNT) and molecularly imprinted polymers. In the first case, SWCNT-modified SPCE as disposable electrochemical sensors was proposed for quick determination of dasatinib in pharmaceutical formulations, demonstrating an excellent boosting effect on the oxidation response of dasatinib. The sensor was able to monitor different dasatinib concentrations with a limit of detection of 0.06 M. In the second paper, the SPE surface was modified with poly tyrosine-chitosan and combined with a self-assembly surface molecular imprinting approach to develop horseradish peroxidase (HRP)-imprinted biosensor. Additionally, the molecularly imprinted electrochemical biosensor for determination of HRP was expanded for the detection of H2O2 as 7 enzymatic substrates. The HRP-imprinted biosensor was determined H2O2 and HRP with detection limits of 2.18 nM and 9.39 × 10-8 mg/ml (2.34 pM), respectively. The last paper was related to developing a DNA-based bio-assay for determining Haemophilus influenza through bioconjugation of citrate-capped silver nanoparticles with pDNA toward target sequences detection. The ultra-sensitive fabricated optical genosensor was detected the synthesized probe (SH-5-AAT TTT CCA ACT TTT TCA CCT GCA T-3') of Haemophilus influenza with great selectivity and sensitivity after hybridization with cDNA with the low limit of quantification of 1 zM (zeptomolar). Finally, the designed genosensor is a significant diagnostic strategy for detecting Haemophilus influenza with great selectivity.Recently, medical organizations and research disciplines have switched to employing affordable biosensors. Biosensors have become more critical in drug development, drug identification, bio-medicine, food safety, security, protection, and ecological research. It has directly contributed to developing specialized and reliable diagnostic equipment that uses biological sensing components as biosensors. Different analytes, biological receptors, and transducer concepts are used in biosensors. In recent years, innovative sensing platforms have been developed employing screen-printed electrodes (SPEs), which are inexpensive, simple, and quick mass production through thick film technology. During my Ph.D., I concentrated on designing, constructing, and validating several SPE-based (bio)sensors to identify biologically active compounds important for healthcare applications, demonstrating their significant potential as a suitable sensing platform. I created (bio)sensors for a variety of analytes, from nucleic acid to small molecules, with specific modification strategies to endow the platforms with selectivity toward the species of interest. I developed disposable electrochemical (bio)sensors in two papers based on modifying with single-walled carbon nanotubes (SWCNT) and molecularly imprinted polymers. In the first case, SWCNT-modified SPCE as disposable electrochemical sensors was proposed for quick determination of dasatinib in pharmaceutical formulations, demonstrating an excellent boosting effect on the oxidation response of dasatinib. The sensor was able to monitor different dasatinib concentrations with a limit of detection of 0.06 M. In the second paper, the SPE surface was modified with poly tyrosine-chitosan and combined with a self-assembly surface molecular imprinting approach to develop horseradish peroxidase (HRP)-imprinted biosensor. Additionally, the molecularly imprinted electrochemical biosensor for determination of HRP was expanded for the detection of H2O2 as 7 enzymatic substrates. The HRP-imprinted biosensor was determined H2O2 and HRP with detection limits of 2.18 nM and 9.39 × 10-8 mg/ml (2.34 pM), respectively. The last paper was related to developing a DNA-based bio-assay for determining Haemophilus influenza through bioconjugation of citrate-capped silver nanoparticles with pDNA toward target sequences detection. The ultra-sensitive fabricated optical genosensor was detected the synthesized probe (SH-5-AAT TTT CCA ACT TTT TCA CCT GCA T-3') of Haemophilus influenza with great selectivity and sensitivity after hybridization with cDNA with the low limit of quantification of 1 zM (zeptomolar). Finally, the designed genosensor is a significant diagnostic strategy for detecting Haemophilus influenza with great selectivity.
Počet záznamů: 1