This work is focused on two-step synthesis of metal core-shell NPs prepared via seeded-growth method exploiting freshly or previously prepared seeds. The as-prepared colloidal systems of NPs are characterized using UV Vis spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), and measurement of zeta-potential values. The direct influence of synthetic parameters and conditions on the characteristic properties of core-shell NPs consisting of the same (Ag-Ag NPs) or different (Au-Ag) plasmonic metal is demonstrated. The ability of the selected colloidal systems to enhance Raman scattering after the interaction with the selected protein, bovine serum, is tested out with surface-enhanced Raman spectroscopy (SERS) at two different excitation laser lines (532 and 785 nm).This work is focused on two-step synthesis of metal core-shell NPs prepared via seeded-growth method exploiting freshly or previously prepared seeds. The as-prepared colloidal systems of NPs are characterized using UV Vis spectroscopy, transmission electron microscopy (TEM), dynamic light scattering (DLS), and measurement of zeta-potential values. The direct influence of synthetic parameters and conditions on the characteristic properties of core-shell NPs consisting of the same (Ag-Ag NPs) or different (Au-Ag) plasmonic metal is demonstrated. The ability of the selected colloidal systems to enhance Raman scattering after the interaction with the selected protein, bovine serum, is tested out with surface-enhanced Raman spectroscopy (SERS) at two different excitation laser lines (532 and 785 nm).