Počet záznamů: 1
Decoding the Antibody Repertoire
Údaje o názvu Decoding the Antibody Repertoire [electronic resource] : High Throughput Sequencing of Multiple Transcripts from Single B Cells / by Brandon DeKosky. Nakladatel Cham : Springer International Publishing : Imprint: Springer, 2017. Fyz.popis XXVIII, 87 p. 34 illus. online resource. ISBN 9783319585185 Edice Springer Theses, Recognizing Outstanding Ph.D. Research, ISSN 2190-5053 Úplný obsah Background -- High-throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire -- In-Depth Determination and Analysis of the Human Paired Heavy and Light Chain Antibody Repertoire -- Paired VH:VL Analysis of Naïve B Cell Repertoires and Comparison to Antigen-Experienced B Cell Repertoires in Healthy Human Donors -- Conclusions and Future Perspectives -- Appendices. Poznámky k dostupnosti Přístup pouze pro oprávněné uživatele Dal.odpovědnost SpringerLink (Online service) Předmět.hesla Medicine. * Human genetics. * Antibodies. * Genetic engineering. * Biochemistry. * Cell biology. Forma, žánr elektronické knihy electronic books Země vyd. Německo Jazyk dok. angličtina Druh dok. Elektronické knihy URL Plný text pro studenty a zaměstnance UPOL kniha
This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening the door for the discovery of new antibodies and the investigation of adaptive immune responses to vaccines and diseases. By designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells, the author directly addresses the limitations to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. Previous methods for high-throughput immune repertoire sequencing have been unable to provide such information. The techniques developed in this thesis have enabled comprehensive investigation of human B-cell repertoires and have been applied for the rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.
Background -- High-throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire -- In-Depth Determination and Analysis of the Human Paired Heavy and Light Chain Antibody Repertoire -- Paired VH:VL Analysis of Naïve B Cell Repertoires and Comparison to Antigen-Experienced B Cell Repertoires in Healthy Human Donors -- Conclusions and Future Perspectives -- Appendices.
Počet záznamů: 1