Záujmový jačmenný gén Hvabc1 je ortológ génu Atwbc19, ktorý v nadexpresii udeľuje transgénnemu tabaku rezistenciu voči antibiotiku kanamycínu. Transformáciou génu Hvabc1 do tabaku bude overená jeho funkcia. Realizáciou navrhnutej klonovacej stratégie bol pripravený expresný vektor obsahujúci záujmový gén Hvabc1. Expresný vektor pCBA::Hvabc1 bol transformovaný do baktérií Agrobacterium tumefaciens. Modifikované baktérie boli použité na transformáciu tabaku (Nicotiana tabacum L., cv. Xanthi). Transformované explantáty a regenerované rastliny boli selektované na médiu s obsahom antibiotika kanamycínu. Tranzientnou transformáciou tabaku bola potvrdená správnosť pripraveného konštruktu.The barley gene of interest, Hvabc1, is orthologous to the gene Atwbc19, which when overexpressed, confers resistance to antibiotic kanamycin in transgenic tobacco. The function of Hvabc1 gene will be determined by transformation into tobacco. The proposed cloning strategy was carried out and expression vector containing the gene of interest Hvabc1, was prepared as a result. The expression vector pCBA::Hvabc1 was transformed into bacteria Agrobacterium tumefaciens. Modified bacteria were used for tobacco (Nicotiana tabacum L., cv. Xanthi) transformation. Transformed explants and regenerated plants underwent a selection process on a medium containing an antibiotic kanamycin. Transient transformation confirmed that the prepared construct was functional.